Effects of culture condition on small RNA transcriptomes in extracellular vesicles released by human bone marrow stromal cells

Various culture media that can rapidly expand bone marrow stromal cells (BMSCs) are currently available. However, the effects of those culture media on the contents of extracellular vesicles released by bone marrow stromal cells have not been fully understood. Using BMSCs from 6 healthy donors were cultured in two different culture media and characterized the small RNA profiles in extracellular vesicles. Total 12 samples were analyzed; BMSCs (#3-#8) were cultured in either DMEM+10% FBS (D) or high perfomance medium (R)