MEK and GSK3β inhibition drives ES cells into a naive pluripotent state irrespective of their global methylation status . Mus musculus

We carry out methyl DNA immunoprecipitation (meDIP), hydroxymethyl DNA immunoprecipitaion (hmeDIP) and H3K27me3 chromatin imminoprecipitation (ChIP) prior to sequencing on the Ion Proton semiconductor sequencing platform to report on the genome-wide epigenetic patterns in J1 mESC and mutant mESCs (hyper active DNMT3b/3l or DNMT1/3a/3b triple knock out) grown under serum conditions or in 2i meadia for 14 days Overall design: Genome-wide patterns were generate on the Ion Proton P1 sequencer.