Effect of K4R and K7R mutation on Aos1 sumoylation and function.
收藏Figshare2016-02-23 更新2026-04-29 收录
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(A) Effect of K4R and K7R mutations on sumoylation of Aos1 in vitro. (B) Effect of Effect of K4R and K7R mutations on Aos1 sumoylation in vivo. Epitope-tagged Aos1-HA in the HF-SUMO strain was purified using anti-HA affinity resins and its sumoylated forms was detected using anti-FLAG Western blot. (C) Growth and sensitivity of various sumoylation-defective aos1 mutants to genotoxic agents. 10-fold dilution of the same amounts of cells were plated and incubated at 30°C for 3 days. The mec1Δ mutant is used as a positive control for its hypersensitivity to genotoxic agents including hydroxyurea (HU), camptothecin (CPT) and UV.
创建时间：
2016-02-23
