Comparison of coding RNA expression in steady-state and in response to IL33 and IL5 stimulation in bone marrow eosinophils from IL5-deficient and wild-type littermates

Aim of the experiment was to assess differences in expression of coding RNA between bone marrow eosinophils arising in IL-5 knock-out mice (C57BL/6-Il5tm1Kopf/J) or wild-type littermates and their response to cytokine stimulation. Bone marrow eosinophils were sorted by FACS submitted to RNA-seq immediately or after 4 hours of stimulation with IL-5 and IL-33 in culture plates.本实验旨在评估IL-5敲除小鼠（C57BL/6-Il5tm1Kopf/J）与野生型同窝仔鼠体内产生的骨髓嗜酸性粒细胞的编码RNA（coding RNA）表达差异，以及这类细胞对细胞因子刺激的应答情况。研究人员通过荧光激活细胞分选（FACS）分离骨髓嗜酸性粒细胞，随后将分选得到的细胞立即进行RNA测序（RNA-seq），或在培养板中以IL-5与IL-33刺激4小时后再进行RNA测序。