Harnessing matrix stiffness to engineer a bone marrow niche for hematopoietic stem cell rejuvenation

RNA and ATAC sequencing data of primary sorting CD45-Ter119-CD31-Scf; GFP+Cxcl12; DsRed+ bone marrow stromal cells ,2D cultured bone marrow stromal cells and 3D cultured bone marrow stromal cells. RNA sequencing data of sorted primary and 3D cocultured Lin-Sca1+C-kit+CD150+CD48+ hematopoietic stem cells from 8-12 weeks and 12-13 months old mice. RNA and ATAC sequencing data of primary sorting CD45-Ter119-CD31-Pdgfra+td-Tomato+ bone marrow stromal cells from young (8 wks), middle aged (12 months) and aged (22-24 months) Lepr-Cre;td-Tomato mice. Overall design: Total RNA from 3 biological replicates of CD45-Ter119-CD31-Scf; GFP+Cxcl12; DsRed+ bone marrow stromal cells,2D cultured bone marrow stromal cells and 3D cultured bone marrow stromal cells. Paired-end sequencing 150bp reads Novaseq (Illumina). ATAC-seq of 3 biological replicates of CD45-Ter119-CD31-Scf; GFP+Cxcl12; DsRed+ bone marrow stromal cells,2D cultured bone marrow stromal cells and 3D cultured bone marrow stromal cells.Paired-end sequencing 150bp reads HiSeq X Ten system (Illumina). RNA-seq of 3 biological replicates of sorted primary and 3D cocultured Lin-Sca1+C-kit+CD150+CD48+ hematopoietic stem cells from 8-12 weeks and 12-13 months old mice.Paired-end sequencing 150bp reads HiSeq X Ten system (Illumina). Total RNA from 3 biological replicates of CD45-Ter119-CD31-Pdgfra+td-Tomato+ bone marrow stromal cells from young (8 wks), middle aged (12 months) and aged (22-24 months) Lepr-Cre;td-Tomato mice. Paired-end sequencing 150bp reads Novaseq (Illumina). ATAC-seq of 3 biological replicates of CD45-Ter119-CD31-Pdgfra+td-Tomato+ bone marrow stromal cells from young (8 wks), middle aged (12 months) and aged (22-24 months) Lepr-Cre;td-Tomato mice. Paired-end sequencing 150bp reads (Illumina).