Sensitivity of TF-1 cells expressing wild-type, ITD and D835Y forms of Flt3 to inhibitors of Fes and Flt3 kinase activity.

TF-1 cells were transduced with retroviruses expressing wild-type (WT), ITD or D835Y forms of Flt3, or an empty vector as negative control. Cells were grown in the presence of the kinase inhibitors shown, and IC50 values were calculated by non-linear curve fitting. The concentration-response curves used to generate these values are shown in Fig 6. Control cells in this experiment were TF-1 cells transduced with an empty retroviral vector.