Additional file 1 of Age, sex, and mating status discrimination in the sand fly Lutzomyia longipalpis using near infra-red spectroscopy (NIRS)

Additional file 1: Figure S1. Principal component analysis (PCA) of non-copulated Lutzomyia longipalpis females at different ages. (a) One (blue squares), 3 (brown triangles), 8 (blue stars), 10 (red circles), 15 (green triangles), and 17 (light blue diamonds) days old; (b) 1 (blue squares), 8 (red circles), and 15 (green triangles) days old; (c) < 8 (blue squares) or ≥ 8 (red circles) days old. Figure S2. Loadings of PCA [principal component 1 (PC – 1)] of non-copulated Lutzomyia longipalpis females at different ages: (a) 1-8-15 days old and (b) < 8 or ≥ 8 days old. Figure S3. Loadings of PCA [principal component 1 (PC – 1)] of non-copulated Lutzomyia longipalpis males at different ages: (a) 1-3-8-10-15-17 days old, (b) 1 8 15 days old, and (c) < 8 or ≥ 8 days old. Figure S4. Principal component analysis (PCA) of non-copulated Lutzomyia longipalpis males at different ages. (a) One (blue squares), 3 (brown triangles), 8 (gray stars), 10 (red circles), 15 (green triangles), and 17 (light blue diamonds) days old; (b) 1 (blue squares), 8 (red circles), and 15 (green triangles) days old; (c) < 8 (blue squares) or ≥ 8 (red circles) days old. Figure S5. Loadings of PCA [principal component 1 (PC – 1)] of copulated Lutzomyia longipalpis females at different ages: (a) 1-8-15 days old and (b) < 8 or > 8 days old, zoomed in. Figure S6. Loadings of PCA (principal component 1 (PC – 1)) of copulated Lutzomyia longipalpis males at different ages: (a) 1-8-15 days old and (b) < 8 or > 8 days old. Figure S7. Principal component analysis (PCA) of copulated Lutzomyia longipalpis at different ages. (a) One (blue squares), 8 (green triangles), and 15 (red circles) days old; (b) < 8 (blue triangles) or > 8 (red circles) days old. Figure S8. Principal component analysis (PCA) of copulated Lutzomyia longipalpis males at different ages. (a) One (blue squares), 8(green triangles), and 15 (red circles) days old; (b) < 8 (blue squares) or > 8 (red circles) days old. Figure S9. Principal component analysis (PCA) of copulated (CP, blue squares) vs. non copulated (NC, red circles) Lutzomyia longipalpis at the same age. (a) Females; (b) males. Figure S10. Principal component analysis (PCA) of females fed on sucrose solution (Suc, blue squares) or sucrose plus blood (Suc_B, red circles). (a) Results keeping females with unlaid eggs, (b) without females with unlaid eggs.