Illumina read metagenomes of Astoria canyon cold seep sediments

This study comprises Illumina read metagenomes from cold seep sediment samples. The samples were collected from site H1867 in Astoria Canyon, Oregon, USA (latitude: 46.240784 N, longitude: 124.602954 W, water depth: 760 m) during the R/V Atlantis cruise AT50-14 on 19 August 2023. 200 g of cold seep sediment and 500 mL artificial anoxic seawater medium with 25 mM magnesium sulfate and 30 mM sodium bicarbonate were used to prepare the sediment slurry. These slurries were then evenly distributed into six 250-mL serum vials and filled up to 100 mL with the anoxic medium. The vials were incubated with CH4 as the energy source. For the lipid stable isotope probing (SIP) experiment, all slurries were pooled and supplied with a new medium with 27 mM DIC. This slurry was distributed in thirteen 250-mL serum vials. Each bottle received 50 mL sediment slurry and 100 mL anaerobic seawater medium, leaving 100 mL gas headspace. Each vial was spiked with either 13C-labeled or non-labeled methane or DIC. DNA was extracted from the SIP incubation experiment using the DNeasy PowerSoil Pro kit (Qiagen) and was sequenced at the LGC Genomics GmbH (Berlin, Germany) as 2 x 150 bp paired-end reads on an Illumina MiSeq sequencing platform.