Deep sequencing of lung cancer primary tumors and serum specimens.

The number of reads detected in each condition is indicated. Adc and SCC stand for adenocarcinoma and squamous cell carcinoma respectively. We selected only reads matching the three following criteria: 1) reads are 17nt long or more; 2) at least 17 consecutive nucleotides match perfectly with the human genome (GRCh37); 3) the nucleotide at the 5′ end of reads maps the human genome. Two types of libraries were built. The short one was built after a gel purification of RNA fragments between 16 to 30 nt long; the long one after a gel purification of RNA fragments between 25 to 50 nt long.