The Neonatal Fc Receptor (FcRn) is a pan-arterivirus receptor

Arteriviruses infect a variety of mammalian hosts, but the receptors used by these viruses to enter cells are poorly understood. We identified the neonatal Fc receptor (FcRn) as an important pro-viral host factor via comparative genome-wide CRISPR-knockout screens with multiple arteriviruses. Using a panel of cell lines and divergent arteriviruses, we demonstrate that FcRn is required for the entry step of arterivirus infection and serves as a molecular barrier to arterivirus cross-species infection. We also show that FcRn synergizes with another known arterivirus entry factor, CD163, to mediate arterivirus entry. Overexpression of FcRn and CD163 sensitizes non-permissive cells to infection and enables the culture of fastidious arteriviruses. Treatment of multiple cell lines with a pre-clinical anti-FcRn monoclonal antibody blocked infection and rescued cells from arterivirus-induced death. Altogether, this study identifies FcRn as a novel pan-arterivirus receptor, with implications for..., , , # The Neonatal Fc Receptor (FcRn) is a pan-arterivirus receptor

[https://doi.org/10.5061/dryad.h9w0vt4s6](https://doi.org/10.5061/dryad.h9w0vt4s6)

Each tab in the .csv file corresponds to the data from a figure in the corresponding manuscript. RRA= Robust Rank Aggregation; DPI= days post infection; PFU= plaque-forming units; FFU= focus forming assay; WT= wild type; FcRn=neonatal Fc receptor; EV= empty vector; YFV= yellow fever virus; SHFV= Simian Hemorrhagic Fever Virus; PRRSV= porcine respiratory and reproductive syndrome virus; EAV= equine arteritis virus; KRCV-1= Kibale Red Colobus Virus-1; PBJV= Pebjah virus; Ct= cycle threshold. All blank cells are left intentionally blank. 

Figure 1A: shows PFU production of SHFV PRRSV on wild-type MA-104 cells inoculated at MOI=0.01, 2 replicates per virus.

Figure 1E: shows RRA scores from the CRISPR-KO survival screen (RRA scores generated in MAGeCK software), with SHFV RRA vs. PRRSV RRA. 

Figure 1 F: shows PFU (or FFU for YFV) production (...