scRNA-seq of mouse sympathetic ganglia cells under control, cold-exposure, and microbiota-depletion conditions

We performed scRNA-seq analysis on sympathetic ganglia, including the superior cervical ganglion (SCG), stellate ganglion (SG), and coeliac–superior mesenteric ganglion (CG-SMG). Comparative analysis of the transcriptomic profiling revealed molecularly distinct cell subtypes. We then performed scRNA-seq analysis in SCG and CG-SMG under control and stressed conditions (cold and microbiota-depletion). These analyses show that sympathetic ganglia undergo dynamic remodeling under stress, with distinct stressors driving ganglion-specific plasticity directly coupled to altered neuronal function.