Time-course global miRNA expression profiling on lung fibroblasts isolated from untreated, bleomycin-treated and silica-treated mice

Lung fibroblasts play a pivotal role in pulmonary fibrosis, a devastating lung diseases, by producing extracellular matrix. MicroRNAs (miRNAs) suppress a lot of genes posttranscriptionally, but the dynamics and the role of miRNAs in activated lung fibroblasts in fibrotic lung has been poorly understood. To elucidate these problems, we performed global miRNA expression profiling of lung fibroblasts of bleomycin- and silica-induced fibrotic lungs Overall design: Global miRNA expression profile of lung fibroblasts isolated from untreated, bleomycin-treated and silica-treated mice on days 7, 14 and 28 post-treatment was generated using Ion Total RNA-seq Kit v2 and Ion PGM sequencer.