Identification of microRNA-668 and microRNA-489 targets by RISC immunoprecipitation

MicroRNAs inhibits their target mRNA translation by forming RISC complex. Immunoprecipitation of RISC will pull down both microRNA and their target mRNAs. To systemetically examine the potential targets of microRNA-668 and microRNA-489, HEK293 cells were overexpressed with negative control, microRNA-668, or microRNA-489 mimics. Both total RNA samples and RNA samples from RISC complex pulled down by Ago-2 immunoprecipitation were examined by RNA-seq. RNAs from 6 groups of samples were analyzed (NC total, mir-668 total, mir-489 total, NC IP, mir-668 IP, mir-489 IP). Each groups has three repeats (n=3).