Engineered cardiac microbundle time-lapse microscopy image dataset

The \"Microbundle Time-lapse Dataset\" contains 24 experimental time-lapse images of cardiac microbundles using three distinct types of experimental testbed of beating lab grown hiPSC-based cardiac microbundles. Of the 24 experimental time-lapse images, 23 examples are brightfield videos, and a single example is a phase contrast video. We categorize the different experimental testbeds into 3 types, where \"Type 1\" includes movies obtained from standard experimental microbundle platforms termed microbundle strain gauges [1,2,3]. We refer to data collected from non-standard platforms termed FibroTUGs [4] as \"Type 2\" data, and \"Type 3\" data represents a highly versatile and diverse nanofabricated experimental platform [5,6].
References:
[1] Boudou T, Legant WR, Mu A, Borochin MA, Thavandiran N, Radisic M, Zandstra PW, Epstein JA, Margulies KB, Chen CS. A microfabricated platform to measure and manipulate the mechanics of engineered cardiac microtissues. Tissue Engineering Part A. 2012 May 1;1..., We include here a brief description of the 3 data types. For \"Type 1\" testbeds, each experimental tissue well consisted of 2 pillars with rectangular cross sections and spherical caps cast from poly(dimethylsiloxane) (PDMS) using a 3D printed mold. Following treatment to promote cell attachment to the spherical caps, hiPSC-CMs, differentiated and purified, were seeded with human ventricular cardiac fibroblasts. And after 5-7 days of seeding, time-lapse videos of tissue contractions were acquired. 
As for \"Type 2\" experimental platforms, arrays of PDMS cantilevers were fabricated by soft lithography. Then, fiber matrices were generated by selective photo-crosslinking of electrospun dextran vinyl sulfone (DVS) fibers and suspended between pairs of cantilevers. After functionalizing the electrospun fiber matrices, iPSC-CMs, differentiated and purified, were patterned onto matrices using microfabricated seeding masks cast from 3D-printed molds. Finally, time-lapse videos of the microtissue’..., Within this dataset, we include 11 examples of \"Type 1\" tissue, 7 examples of \"Type 2\" tissue, and 6 examples of \"Type 3\" tissue, totaling to 24 different examples of these experimental data. In addition to the raw videos shared in \".tif\" format, we include the tissue masks, whether generated automatically via our computational pipeline [7] or manually via tracing in ImageJ [8], that were used to run the \"MicroBundleCompute\" software [7] for analyzing these data. These masks are included within the \"masks\" subfolders, where each mask text file is a two-dimensional array in which the tissue domain is denoted by “1” and the background domain is denoted by “0”. We include the \"mask.tif\" files for visualization purposes only.
In brief, this dataset was used to showcase the functionality of the \"MicroBundleCompute\" analysis software [7] including pillar tracking and analysis of heterogeneous displacement and strain fields. To reproduce the results shown in [9], the manuscript introducing the..., # Microbundle Time-lapse Dataset

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Brief summary of dataset contents, contextualized in experimental procedures and results.

## Abstract

The \"Microbundle Time-lapse Dataset\" contains $24$ experimental time-lapse images of cardiac microbundles using three distinct types of experimental testbed of beating lab grown hiPSC-based cardiac microbundles. Of the
$24$ experimental time-lapse images, $23$ examples are brightfield videos, and a single example is a phase contrast video. We categorize the different experimental testbeds into $3$ types, where \"Type 1\" includes movies obtained from standard experimental microbundle platforms termed microbundle strain gauges [1,2,3]. We refer to data collected from non-standard platforms termed FibroTUGs [4] as \"Type 2\" data, and \"Type 3\" data represents a highly versatile and diverse nanofabricated experimental platform [5,6].

## Methods:

We include here a brief description of the 3 data types. For \"Type 1\" testbeds, each experimental tissue w...