Genome-wide profiling for h3k4me3, h3k27me3, and h3k9me3 in Uhrf1hepKO mouse livers

This study was carried out to investigate potential changes in the genome-wide distribution of h3k4me3, h3k27me3, and h3k9me3 in Uhrf1hepKO mouse livers compared to wild-type. ChIP was carried out to pull down h3k4me3, h3k27me3, or h3k9me3 bound chromatin from nuclei isolated from wild-type and Uhrf1hepKO liver tissues. This chromatin fraction was then digested using micrococcal nuclease to release mononucleosomes. DNA fragments purified from nucleosomes were then used to generate libraries and sequenced.