Expression data from cultured spermatogonia established from Cdkn1c conditional knockout mouse testes

Mice lacking Cdkn1c (p57) in spermatogonia were found to be congenitally infertile due to spermatogonia depletion. Transplantation experiments showed that Cdkn1c deficiency compromised spermatogonial stem cells (SSC) activity. SSCs undergo self-renewal division to sustain spermatogenesis, and it is possible to derive germline stem (GS) cell cultures by supplementing GDNF and FGF2. We examined the influence of Cdkn1c depletion on gene expression of GS cells. In this dataset, we include the expression data obtained from cultured spermatogonia (GS cells) derived from Cdkn1c(p57) floxed/floxed mice. Cdkn1c(p57) floxed/floxed GS cells were treated with adenovirus cre recombinase. Each group contains 3 biological replicates. Overall design: GS cells were established from testes of 10 day old mice of C57BL/6 and 129 strains. Total RNA samples were extracted with TRIzol reagent (Invitrogen) and purified using the RNeasy cleanup system (QIAGEN, Valencia, CA). cDNA libraries were generated using a TruSeq stranded mRNA library preparation kit (Illumina, San Diego, CA). Sequencing was performed using NextSeq550 (Illumina) with a single-read sequencing length of 76 bp. 6 Total samples were analyzed.