Whole genome ChIP-seq of histone H3 threonine 11 phosphorylation in Saccharomyces cerevisiae

We used ChIP-seq to determine the whole-genome enrichment of histone H3 threonine 11 phosphorylation (H3 T11ph) during Saccharomyces cerevisiae meiosis. S. cerevisiae SK1 cells were synchronized for meiotic entry and 3 and 4 hour meiotic samples were obtained. As H3 T11ph is dependent on the formation of meiotic double strand breaks (DSBs), a negative control ChIP-seq sample was obtained from a strain lacking DSBs (spo11-yf). Concurrently, ChIP-seq was carried out for histone H3 as a control for comparision.本研究采用染色质免疫共沉淀测序（ChIP-seq）技术，检测酿酒酵母（Saccharomyces cerevisiae）减数分裂过程中组蛋白H3苏氨酸11磷酸化（H3 T11ph）的全基因组富集情况。我们对酿酒酵母SK1菌株的细胞进行同步化处理以使其进入减数分裂，并分别收集减数分裂培养3小时和4小时的样本。由于H3 T11ph的形成依赖于减数分裂DNA双链断裂（double strand breaks，DSBs）的发生，我们从缺失DSBs的菌株（spo11-yf）中获取了ChIP-seq阴性对照样本。同时，我们还针对组蛋白H3开展了ChIP-seq实验，作为对照以用于结果比对。