Single-cell RNA-sequencing of umbilical cord blood reveals impaired fetal immune homeostasis by gestational diabetes mellitus

Gestational diabetes mellitus (GDM), the most prevalent metabolic disorder during pregnancy, has long-term risks of metabolic diseases that might persist in adulthood. However, the underlying mechanisms remain unclear. Here, we profiled 78,767 cord blood mononuclear cells (CBMCs) from GDM and healthy mothers' fetuses by single-cell RNA sequencing (scRNA-seq). Overall design: Single-cell suspensions from cord blood mononuclear cell of 4 GDM mothers and 3 healthy mothers were prepared and single cell RNA-seq libraries were generated using 3' V2 chemistry kit on Chromium Single cell controller (10x Genomics).