Towards Understanding the Nuclear Organisation of Human Pluripotency Transcription Factors at High Spatial Resolution.

Human pluripotent stem cells are an important cell type during early development, possessing the ability to differentiate into the various cell types that compose the adult body. Establishment and maintenance of pluripotency is determined, in part, by transcription factor proteins. Transcription factors help to define cellular identity through their interactions with DNA and other nuclear proteins. However, the spatial arrangement of pluripotency transcription factors within the nucleus has thus far been underexplored. Using advanced fluorescence microscopy, the subcellular organisation of transcription factors was characterised, revealing that the nuclear organisation of key pluripotency transcription factors was distinct across different pluripotency states.