Single-cell mRNA profiling reveals changes in solute carrier expression and a metabolic switch during zebrafish pronephros development

Developing organisms need to adapt to environmental variations as well as to rapid changes in substrate availability and energy demands imposed by fast-growing tissues and organs. Little is known about the adjustments that kidneys undergo in response to these challenges. We performed single-cell RNA sequencing of zebrafish pronephric duct cells to understand how the developing kidney responds to changes in filtered substrates and intrinsic energy requirements. Single cell RNA sequencing was performed on pronephric cells from 1, 2 and 3-day old zebrafish larvae.