Expression data from splenic T cells at day7 during MOG-induced mouse EAE model. Expression data from splenic T cells at day7 during MOG-induced mouse EAE model

Regulatory T cells (Tregs) are indispensable for maintaining immunological homeostasis and preventing autoimmune diseases. IRE1a is an ER-resident transmembrane protein kinase/endoribonuclease that initiates a critical signaling branch of the unfolded protein response (UPR). We aim to search for genes change of Tregs and Teffs from WT (Foxp3Cre)and IRE1a-/-(KO) mice in the context of MOG-induced experimental autoimmune encephalomyelitis. Our results reveal an important mechanism that links the IRE1a branch of the UPR to experimental autoimmune encephalomyelitis(EAE) model, showing IRE1a affects Tregs suppressive function via mediating HDAC9 upregulation. Our findings suggest that Treg IRE1a is a valuable therapeutic target against multiple sclerosis and other autoimmue diseases. Overall design: Transcriptome analysis of splenic Tregs (CD4+CD25hiYFP+) and Teffs (CD4+CD25-YFP-) of WT and IRE1a-/-(KO) at EAE7d