mRNA expression of fibronectin in FBLN-1 AO transfected asthmatic ASM cells in the presence of TGFβ measured by quantitative real time PCR.

Cells were derived from asthmatic (n = 6) volunteers and treated with Effectene® only, or transfected with 200 nM scrambled AO or FBLN-1C specific AO for 72 h. Cells were treated with either 0.1% BSA or 10 ng/mL TGFβ for 8 h. Expression levels were normalised to the housekeeping gene 18S. Data are expressed as fold change compared with 0.1% BSA values and shown as mean ± SEM [P value]. P values compared with their respective non-stimulated (0.1% BSA) values.