rCRUX Generated Baker Dlp1.5-H/Oordlp4 Reference Database

rCRUX generated reference database using NCBI nt blast database downloaded in December 2022. Primer Name: Scott Baker Dlp1.5-H/Oordlp4<br> Gene: D loop<br> Length of Target: 350-390<br> get_seeds_local() minimum length: 245<br> get_seeds_local() maximum length: 495<br> blast_seeds() minimum length: 205<br> blast_seeds() maximum length: 455<br> max_to_blast: 100<br> Forward Sequence (5'-3'): TCACCCAAAGCTGRARTTCTA<br> Reverse Sequence (5'-3'): GCGGGTTGCTGGTTTCACG<br> Reference: Baker, C. S., Steel, D., Nieukirk, S., &amp; Klinck, H. (2018). Environmental DNA (eDNA) from the wake of the whales: Droplet digital PCR for detection and species identification. Frontiers in Marine Science, 5, 133. https://doi.org/10.3389/fmars.2018.00133 We chose default rCRUX parameters for <em>get_blast_seeds</em>() of percent coverage of 70, percent identity of 70, evalue 3e+7, and max number of blast alignments = '100000000' and for <em>blast_seeds</em>() of coverage of 70, percent identity of 70, evalue 3e+7, rank of genus, and max number of blast alignments = '10000000'.