Ribosome profiling of mitotic exponential cells expressing either 2XDbp1, 1XDbp1, or Ded1

We replaced the DED1 ORF with DBP1 and inserted this either twice or four times in a diploid cell. We determined that with twice as much DBP1 expressable, the same amount of protein was made as for DED1, and there were few changes in gene expression. The transcripts that were translated better with DED1 were ones with highly structured 5' leaders, consistent with Dbp1 acting as a less effective initiation helicase than Ded1. Overall design: We inserted twice as many copies of DBP1 in the genome to match helicase levels and determine helicase specificity