Expression and regulation of corticotropin-releasing factor receptor type 2 beta in developing and mature mouse skeletal muscle

The purpose of the study was to further understand the molecular mechanisms mediating the effect of Crhr2 signaling on insulin sensitivity in skeletal muscle. To that end we compared the gene expression profile of skeletal muscle obtain from both Crhr2 KO and WT littermates using gene expression microarray. RNA was extracted from skeletal muscle of 4 Crhr2 KO and 4 WT littermates. RNA from 2 KO or 2 WT mice was pooled and analyzed using Affymetrix U74Av2 GeneChips.本研究旨在进一步阐明Crhr2信号通路（Crhr2 signaling）对骨骼肌胰岛素敏感性产生调控作用的分子机制。为此，本研究利用基因表达微阵列（gene expression microarray）技术，对比分析了Crhr2基因敲除（knockout, KO）与野生型（wild type, WT）同窝小鼠的骨骼肌基因表达谱。研究人员从4只Crhr2基因敲除小鼠及4只野生型同窝小鼠的骨骼肌中提取RNA，将2只基因敲除小鼠或2只野生型小鼠的RNA样本混合后，采用Affymetrix U74Av2基因芯片完成检测分析。