Transcriptional profiling of Wilms tumor via single nuclei RNA sequencing

Wilms tumor, or nephroblastoma, is the most common pediatric kidney cancer, and is known to arise from renal precursor cells in the fetal kidney. Given that single nuclei RNA-sequencing generates gene expression profiles of diverse cell types within a heterogeneous tissue, here we sought to utilize this technology to profile blastema, stroma, and epithelial components of favorable histology Wilms tumor samples. Frozen, unfixed samples were biobanked at the time of surgical resection/nephrectomy. Nuclei isolation was performed for 10x genomics single nuclei RNA-seq, targeting 5000 nuclei with 50K reads/nuclei from each sample.