Analyses of Methamphetamine-Exposed and HIV- infected Human Monocyte-Derived Macrophages (hMDM). Analyses of Methamphetamine-Exposed and HIV- infected Human Monocyte-Derived Macrophages (hMDM)

Human monocyte-derived macrophages (hMDM) were differentiated in the presence and absence of methamphetamine. Post differentiation, hMDMs were infected with human immunodeficiency virus (HIVADA). To investigate the effects of Meth exposure on infected macrophages day 5 post HIVADA infection, RNA was isolated from HIVADA hMDMs and sequenced using Next Generation Sequencing performed on the Illumina NextSeq550. Overall design: Macrophages differentiation in the presence or absence of Methamphetmine, infected with HIV, treated or not with Methamphetamine. RNA was isolated from HIV-infected macrophages and subjected to RNAseq