five

Efficiency of the versatile FAST vector assembly system.

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NIAID Data Ecosystem2026-03-08 收录
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https://figshare.com/articles/dataset/_Efficiency_of_the_versatile_FAST_vector_assembly_system_/1040968
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aCloning efficiency is based on 3 transformants for each assembled construct and calculated as the number of transformants that contained all inserts in correct orientation divided by the number of screened transformants. bAssembly of five double-stranded PCR fragments, and one oligonucleotide-based part; cReligation of the backbone vector fragment.
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2014-05-30
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