Mapping of rRNA 2'-O-methylation of the RibCancer cell line panel

Somatic and congenital deletions and point mutations in ribosomal proteins (RPs) are recurrent in cancer. Yet, their role in translation and thereof on oncogenesis remains poorly understood. To assess their functional role in translation, we performed an integrated multiomics genome-wide analysis (transcriptome, translatome, proteome) on an isogenic cell line library (the RiboCancer panel) modeling the most frequent RP mutations (Rpl5+/-, Rpl11+/- , Rpl22+/- , Rpl22-/-, Rpl10 R98S, Rps15 P131S and Rps15 H137Y) within the mouse pre B cell Ba/F3 cell line model. Ribosomal RNA chemical modifications have recently emerged as key actors of the translational activity of ribosomes and of the regulation of cellular translation. Here we have mapped 2'-O-methylation, the most abundant rRNA modification using RiboMethSeq, a quantitaitive NGS based mapping method.