Evaluating data acquisition and interpretation using diagnostic plot generated by DO-MS. (a) Distributions of intensities for precursors quantified in all displayed experiments. (b) The distributions
We combine genome-wide CRISPR knockout screening with variant abundance by massively parallel sequencing (VAMP-seq) to identify components regulating PQC of Parkin missense variants. We find that HSP9
Photomicrographs of crystal violet stained parental (P) Min6 cells following transduction with a blasticidin resistance-marked lentivirus expressing either green fluorescent protein (GFP) or a fusion
Hsp60 chaperonins and their Hsp10 cofactors assist protein folding in all living cells, constituting the paradigmatic example of molecular chaperones. Despite extensive investigations of their structu
