RNA sequencing of soybean under mild drought stress

Soybean (Glycine max L. Merr.), which is the most economically important dicot crop worldwide, is severely constrained by abiotic stresses, particularly drought. Mild drought stress is a major detriment to productivity in soybean. To understand the mechanism in response to such mild drought stress in soybean in the actual field, we artificially induce mild drought stress that decreases the soybean growth and yield using ridges in the field. To investigate gene expression specific to mild drought conditions in field-grown soybean plants, we performed RNA-sequencing (RNA-seq) of fully expanded V2 leaves of soybean (Williams82) grown under the flat (control) and ridge (mild drought).