Identification of gene expression changes induced by ligands for RXR and its partners in differentiating human monocyte-derived dendritic cells. Homo sapiens

Differentiating human dendritic cells were stimulated for 12 hours with RXR agonists (LG268, 9CRA, R- and S-9CDHRA) or agonists for RXR partners including GW3965 (LXRα/β panagonist), RSG (PPARγ agonist), and GW1516 (PPARδ agonist) and AM580 (RARa agonist). The gene expression changes were detected globally by Affymetrix Human Genome U133 Plus 2.0 Arrays. Overall design: Human monocytes were obtained by Ficoll gradient centrifugation followed by immunomagnetic cell separation, and were cultured in the presence of GM-CSF and IL-4. Differentiating DCs were stimulated 18 h after plating by various nuclear receptor agonists or vehicle (DMSO:EtOH) for 12h. The ligands were used in the following concentrations: 10 μM R-9CDHRA, 10 μM S-9CDHRA, 1 μM 9cisRA, 100 nM LG100268 (LG268), 1 μM GW3965, 1 μM Rosiglitazone (RSG), 1 μM GW1516, 100 nM AM580. Experiments were performed in biological triplicates.