Single-nuleus RNA-sequencing of septal cells during postnatal development

Purpose: The goal of this study is to use snRNA-seq to identify the diversity of cell types derived from Nkx2.1 lineage and non-Nkx2.1 lineages in the postnatal developing septum, and examine the cell trajectories, especially glial cells in the develping septum; Methods: The septum of P0, P3, P7,P14,P21 Nkx2.1Cre;SunGFP were collected, and nuclei of the septum cells were isolated, then GFP+ and GFP- nuclei were sorted out and followed by 10X genomics sequencing; Results: we found diversity of astrocytes in the septum based on unique molecular profiles which follows the lineage separation, and we also observed the distinct developmental trajectories of subpopulation of astrocytes. Overall design: We perform 10X sequencing on Nkx2.1 and non-Nkx2.1-derived cells in the setpum during different postnatal stages.