10x Lacrimal Gland scRNA seq data matrix

Isolated Lacrimal gland for Single Cell RNA sequence data from E16 and P4
mice. These are the raw read count matrices along side the barcode for the
cells and genes that span the sparse matrix. These results are published
in Development: Defining epithelial cell dynamics and lineage
relationships in the developing lacrimal gland.Abstract:The tear producing
lacrimal gland is a tubular organ that protects and lubricates the ocular
surface. While the lacrimal gland possesses many features that make it an
excellent model to understand tubulogenesis, the cell types and lineage
relationships that drive lacrimal gland formation are unclear. Using
single cell sequencing and other molecular tools, we reveal novel cell
identities and epithelial lineage dynamics that underlie lacrimal gland
development. We show that the lacrimal gland from its earliest
developmental stages is composed of multiple subpopulations of immune,
epithelial, and mesenchymal cell lineages. The epithelial lineage exhibits
the most substantiative cellular changes, transitioning through a series
of unique transcriptional states to become terminally differentiated
acinar, ductal and myoepithelial cells. Furthermore, lineage tracing in
postnatal and adult glands provides the first direct evidence of unipotent
KRT5+ epithelial cells in the lacrimal gland. Finally, we show
conservation of developmental markers between the developing mouse and
human lacrimal gland, supporting the use of mice to understand human
development. Together, our data reveal critical features of lacrimal gland
development that have broad implications for understanding epithelial
organogenesis.