Transcriptome analysis of Cucumis sativus in drought stress

The study utilizes two contrasting cucumber lines: WBC-23-2, which is drought-tolerant, and DGPC-59, which is drought-sensitive. The plants were exposed to drought conditions using a polyethylene glycol (PEG) solution, and after 48h of stress treatment, 10 leaves from the drought-treated plants and 10 leaves from the plants maintained under control conditions under a hydroponic medium from each replication were sampled randomly from both cultivar (a total of 12 samples) in three biological replicates Total RNA was extracted from the leaves of two contrasting genotypes for transcriptome analysis in triplicate. Total RNA was extracted using the TRIzol reagent (Invitrogen, Waltham, MA, USA). The concentration (optical density 260 nm/280 nm ratio) and quality (optical density 260 nm/230 nm ratio) of the extracted RNA were determined using Bio-analyzer (Agilent, UK). The RNA sequencing was performed using Illumina HiSeq X10.