Richard Allen (University of Hawaii) (2011) CIL:13157, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

Basal bodies are supported by three fiber systems, a striated kinetodesmal fiber that arises from the proximal end of the basal body and curves upward and anteriorly within the longitudinal ridge to the cell’s right side of a row of basal bodies. Extending posteriorly and also curving up into the longitudinal ridge is a ribbon of postciliary microtubules. The kd may extend past several more anterior basal bodies but the postciliary ribbon does not extend beyond the next posterior basal body or pair of basal bodies in the kinety. Only the more posterior of the two basal bodies within a single depression is associated with the ends of these two accessory structures. An indentation of the plasma membrane, the parasomal sac, lies to the cell's right of each basal body or pair of basal bodies. TEM taken on 1/5/73 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 19,500X. Bar = 0.2µm. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. An unprocessed, high resolution version of this image (CIL:12068) is in the library and available for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

基体结构由三个纤维系统所支撑，其一为来自基体近端并沿纵向脊向上前方弯曲至基体行右侧的条纹状动粒纤维。紧随其后并同样弯曲进入纵向脊的是一束后睫状微管。动粒可能延伸至多个更前方的基体，然而后睫状带并不会超出紧邻其后或同一动粒行中的下一个基体对。在单一凹陷内，仅较后方的两个基体与这两种辅助结构的末端相联系。细胞右侧每个基体或基体对的附近，存在一血浆膜的凹陷，即旁体囊。1973年1月5日，R. Allen 使用日立 HU11A 仪器在 75kV 电压下进行的透射电子显微镜（TEM）实验，拍摄了该图像。负片放大倍数为 19,500 倍，标尺长度为 0.2 微米。负片经打印至纸张，并扫描至 Photoshop。此数字化图像可用于定性分析。未经处理的、高分辨率的图像版本（CIL:12068）存档于图书馆，可供定量分析使用。采用标准戊二醛固定法，随后用四氧化锇处理，酒精脱水并嵌入环氧树脂中。使用微切刀制备的切片厚度约为 75 纳米。更多详细信息请访问（http://www5.pbrc.hawaii.edu/allen/）。