Host reactions of some isolates of Turnip mosaic virus from Orchis spp. and other hosts.

aReaction of inoculated leaves/uninoculated upper leaves. At least three plants were inoculated. BSS; Black stem streak, CS; Chlorotic spot, D; Dead, LD; Leaf deformation, LI; Latent infection, M; Mosaic, Mo; Mottle, ND: Not detected, NLL; Necrotic local lesion, NS; Necrotic spot, NR; Necrotic ringspot, PSF; Pod set failure, St; Stunting, VC; Vein clearing, -; No infection, (); Occasionally. All the leaves were examined for Turnip mosaic virus (TuMV) infection by DAS-ELISA. All the absorbance values at 405 nm of DAS-ELISA were greater than 1.0 2.5 hrs after adding substrate.bOS and ORM isolates are aphid transmissible but OM-N isolate is not.cTypical symptom for most of TuMV brassica isolates.dViruses were detected only in upper two leaves, and not in the third.