BTLA Signaling in Conventional and Regulatory Lymphocytes Coordinately Tempers Humoral Immunity in the Intestinal Mucosa. Stienne et al

These datasets include analysis of how BTLA regulates transcriptional pathways in lymphocytes, and how this impacts the makeup of the intestinal microbiota in vivo. We hypothesize that anti-BTLA activates discrete transcriptional changes in diverse lymphocyte populations. Bioinformatic analyses here show RNAseq (mouse) and Nanostring (human) data of how anti-BTLA activates inhibitory signaling in mouse and human T cells and B cells. We additionally hypothesize that these transcriptional changes will result in functional changes to T and B cell collaboration in the germinal center reaction, including changes in mucosal IgA production, that impacts the makeup of the microbiome (in mouse). Here we have also analyzed microflora in anti-BTLA and control treated animals, as well as in animals with cell specific deletions in BTLA in T cells or B cells.