Unravelling the developmental roadmap towards human brown adipose tissue. Unravelling the developmental roadmap towards human brown adipose tissue

We have developed and characterised functionally and molecularly a new chemically-defined protocol for the differentiation of human pluripotent stem cells (hPSCs) into brown adipocytes (BAs) that overcomes current limitations This protocol recapitulates step-by-step the physiological developmental path of human BAT. The BAs obtained expresses brown adipocyte and thermogenic markers, are insulin sensitive, and responsive to β-adrenergic stimuli. This new protocol is scalable, enabling the study of human BAs at early stages of development. Overall design: mRNA profiles of the human embryonic stem celll line H9 and the human iPS cell line KOLF2-C1 at different stages of differentiation. Days of differentiation included D0, D2, D4. D8, D10, D12, D20 and D25 or D30. For H9 we have 3 biological replicates and for the KOLF2-C1, 5 biological replicates.