Table S1 - Two-Component Systems Are Involved in the Regulation of Botulinum Neurotoxin Synthesis in Clostridium botulinum Type A Strain Hall

Optimization of quantitative PCR amplification. PCR efficiencies were calculated from a standard curve performed with a serial 10-fold dilution of chromosomal Hall DNA and a temperature gradient ranging from 51 to 68°C for each primer pair targeting bont/A, ntnh, rpob, botR/A, and ha34 genes. Annealing temperature of 51°C was used in qRT-PCR for ha34 and botR/A genes, and 65°C for bont/A, ntnh, and rpob genes. These annealing temperatures yielded a PCR efficiency close of 100% in the largest range of DNA detection. (DOC)