Bulk RNA sequencing for various tissues using SENCR-BAC transgenic mice

SENCR is a vascular-restricted long noncoding RNA specifically expressed in humans. To investigate its biological role in vivo, we generated a transgenic mouse line by introducing a ~220 kb genomic locus encompassing SENCR and its antisense neighbor FLI1, using a bacterial artificial chromosome (BAC clone: RP11-744N12) in combination with PiggyBAC technology. To assess whether SENCR-BAC mice recapitulate the human tissue-specific expression pattern, we collected multiple tissues, including the aorta, heart, brain, kidney, liver, lung, spleen, and stomach, and performed bulk RNA sequencing to evaluate SENCR expression levels across different tissues. The clean reads were mapped to genome using human genome database, but not mouse genome database, due to the objective of this experiment is focused on comparing the level of SENCR, which is human specific. The aorta, brain, heart, kidney, liver, lung, spleen, and stomach were collected in biological triplicates for bulk RNA-seq.