Mechanism study of VEGF-B maintaining genomic stability in HUVECs using mRNA sequencing. Mechanism study of VEGF-B maintaining genomic stability in HUVECs using mRNA sequencing

The goal of this study is to dig out DNA damage pathways mediated by VEGF-B. Using RNA-seq differential expression analysis, we observed genes involved in genomic stability maintainence were upregulated in VEGF-B treated HUVECs. Increased expression of 7 key DNA damage processing genes was confirmed by qRT–PCR. Our study displays the transcriptomic change of HUVECs in the early stage of VEGF-B treatment without strong stress and suggests that VEGF-B signal upregulates genes of DNA damage processing pathway in HUVECs. Overall design: Collecting HUVECs treated with BSA or VEGF-B after 6 hours for mRNA sequencing and comparing the expression changes between the treatments.