果胶多糖的结构与性能

以林业锥栗总苞为原料进行热水浸提，经过醇沉、脱色素、脱蛋白以及DEAE-Sepharose Fast Flow离子交换柱和Sephadex-G50凝胶柱的分离纯化得到一种阿拉伯半乳聚糖组分（CHIP3），再对该组分进行理化性质及抗氧化活性分析。结果表明：SJP-2总糖含量为 98.01%，不含蛋白，其纯度为99.61%，是一个低分散度，分子量较为均一的多糖，分子量为2.44×104g/mol；HPLC分析得知CHIP3为一种杂多糖，其单糖组成及比例为甘露糖（10.7%）：鼠李糖(8.7%):半乳糖醛酸（38.21%）：半乳糖（13.75%）：阿拉伯糖（28.63%）；核磁共振及甲基化结果显示该组分主链为1→4链接的半乳糖醛酸和1→2链接的鼠李糖。

Using forestry-harvested Chinese chestnut (Castanea henryi) involucres as raw material, hot water extraction was performed, followed by alcohol precipitation, decolorization, deproteinization, and sequential separation and purification using DEAE-Sepharose Fast Flow ion exchange column and Sephadex-G50 gel filtration column to obtain an arabinogalactan fraction designated as CHIP3. Subsequently, the physicochemical properties and antioxidant activity of this fraction were analyzed. The results demonstrated that: SJP-2 had a total sugar content of 98.01%, was protein-free, with a purity of 99.61%. It was a polysaccharide with low dispersity and relatively homogeneous molecular weight, with a molecular mass of 2.44×10⁴ g/mol. HPLC analysis revealed that CHIP3 was a heteropolysaccharide, with its monosaccharide composition and molar ratio as follows: mannose (10.7%), rhamnose (8.7%), galacturonic acid (38.21%), galactose (13.75%), and arabinose (28.63%). Nuclear magnetic resonance (NMR) spectroscopy and methylation analysis showed that the main chain of this fraction consisted of 1→4-linked galacturonic acid residues and 1→2-linked rhamnose residues.

搜集汇总

背景与挑战

背景概述

该数据集聚焦于从林业锥栗总苞中提取的果胶多糖（CHIP3）的结构与性能分析，通过实验揭示了其高纯度、低分散度的理化特性，包括分子量、单糖组成和主链结构。数据集属于'人工林非木质林产资源高质化利用技术创新'项目，提供了一份详细的实验研究报告，旨在支持植物学领域的高质化资源利用研究。