Data from: The translational landscape of the splicing factor SRSF1 and its role in mitosis

The shuttling Serine/Arginine rich (SR) protein SRSF1 (previously known as
SF2/ASF) is a splicing regulator that also activates translation in the
cytoplasm. In order to dissect the gene network that is translationally
regulated by SRSF1, we performed a high-throughput deep sequencing
analysis of polysomal fractions in cells overexpressing SRSF1. We
identified approximately 1,500 mRNAs that are translational targets of
SRSF1. These include mRNAs encoding proteins involved in cell cycle
regulation, such as spindle, kinetochore and M phase proteins, which are
essential for accurate chromosome segregation. Indeed, we show that
translational activity of SRSF1 is required for normal mitotic
progression. Furthermore, we found that mRNAs that display alternative
splicing changes upon SRSF1 overexpression are also its translational
targets; strongly suggesting that SRSF1 couples pre-mRNA splicing and
translation. These data provide insights on the complex role of SRSF1 in
the control of gene expression at multiple levels and its implications in
cancer.