Intra- and inter-individual genetic differences in gene expression (C57BL/6J vs DBA/2J)

Genetic variation is known to influence the amount of mRNA produced by a gene, but the effects in different tissues is unclear. We studied gene expression levels in whole brain, kidney or liver, comparing 8 week old, male C57BL/6J to DBA/2J mice. Genes differentially expressed due to genetic background in one tissue were not differentially expressed in other tissues, suggesting that genetic variation of gene expression is tissue specific in inbred mice. Data used in a comparison to the BXD panel of mice in GSE8355, GSE8356, GSE6621 and to SJL/J in GSE8358. Each sample is a single hybridisation from a common pool of C57BL/6J or DBA/2J. Two-colour glass arrays were used. No dye swaps were employed. Data from whole brain, kidney or liver.已知遗传变异可影响基因转录产生的信使RNA（mRNA）水平，但该效应在不同组织中的具体表现尚未明确。本研究针对全脑、肾脏及肝脏组织的基因表达水平展开分析，对比了8周龄雄性C57BL/6J与DBA/2J两种近交系小鼠的表达差异。仅在单一组织中因遗传背景出现差异表达的基因，在其余组织中并无显著表达差异，这表明近交系小鼠中基因表达的遗传变异具有组织特异性。本研究数据还与GSE8355、GSE8356、GSE6621数据集内的BXD小鼠参考群体（BXD panel）数据，以及GSE8358数据集内的SJL/J小鼠数据开展了对比分析。每个样本均取自C57BL/6J或DBA/2J小鼠的统一混合样本池，仅完成单次杂交反应。实验采用双色玻璃微阵列芯片（two-colour glass arrays）技术，未设置染料交换对照。本次分析涵盖全脑、肾脏及肝脏三类组织的表达数据。