Profiling of circular RNAs in gastric cancer tissues and adjacent normal tissues

Purpose: To investigate the role and mechanism of circRNAs in gastric cancer. Methods: RNA-seq of ribosomal RNA-depleted total RNA and qRT-PCR were performed to screen differential expressed circRNAs between paired gastric cancer tissues and adjacent normal tissues. Results: A total of 57623 distinct circRNAs were identified in these samples, and 35120 of these circRNAs contained at least two independent back-spliced reads in at least two samples. Compared with circBase, we found that there were 29007 matched circRNAs and 28616 novel circRNAs in our study. Gastric circRNA profiles of paired gastric cancer tissues and adjacent normal tissues were generated by deep sequencing, using Illumina HiSeq Xten.