Expression analysis of A375 melanoma cells with silenced BMI-1 or not

Our studies demonstrate that BMI-1 plays a crucial role in the EMT process and drug resistance in melanoma. Silencing Bmi-1 significantly inhibites the aggressive behavior and improves the chemosensitivity to all-trans retinoic acid on melanoma cells. We compared the different genes expression of A375-vector and A375-BMI-1 cells for exploring the underlying mechanisms of BMI-1 regulation on melanoma. A two chip study using total RNA recovered from two cultured cells, A375-vector and A375-BMI-1, which were transfected with empty lentivirus and target sequences shBMI-1. Silenced BMI-1 in A375-shBMI-1 cells was confirmed by real-time PCR and western blot analysis comparing to the control cells.