Effect of CWG-cPIP on alternative splicing defects in CUG mouse brain

CWG-cPIP targets CAG/CTG (CWG) triplet repeat DNA to inhibit the transcription in a repeat length-dependent manner. To investigate effects of CWG-cPIP on alternative splicing defects in the brain of CUG300 mice, which we developed as a model of DM1, CWG-cPIP (83 ug/kg) was injected into mouse hippocampus. RNA sequencing analysis was performed using mouse hippocampus 10 days later. Alternative splicing analysis of RNA sequencing data for the mouse hippocampus. The groups consist of 1) CUG10 + vehicle (n = 1 mouse); 2) CUG300 + vehicle (n = 1 mouse); 3) CUG300 + CWG-cPIP (n = 1 mouse).