The secretome of the rumen fluke, Calicophoron daubneyi

Helminth parasites secrete molecules in soluble form (i.e the secretome) or packaged into extracellular vesicles (EVs) as a means of exporting effector molecules into the host microenvironment. Once released, the parasite-derived molecules can trigger a range of biological effects including modulation of host immunity. While the secretome and molecular cargo of EVs have been characterised in many parasites, little is known about the molecules released by the rumen fluke, Calicophoron daubneyi – an emerging infection of livestock in Western Europe. Here we used a mass spectrometry-based proteomics approach to characterise the secretome of the infective newly-excysted juvenile (NEJ) stage and adult flukes. We also profile the proteome of two sub-populations of EVs (termed 15K and 120K EVs) released by the adult flukes.