Rat hindlimb arteries: Shunt versus Control

Arteriogenesis is defined as the development of a collateral circulation, bypassing an arterial occlusion. We have previously demonstrated that chronically elevated fluid shear stress completely normalized blood flow in femoral artery ligated rabbits. This was realized by a surgical intervention: the creation of an arterio- venous anastomosis (shunt) between the femoral artery and the accompanying vein. The aim of this study was a gene profiling approach in order to identify differentially expressed genes during collateral growth in the shunt model when compared to pre-existent collaterals (sham).动脉生成（Arteriogenesis）被定义为构建侧支循环以绕过动脉闭塞的生物学过程。我们此前的研究已证实，慢性升高的流体剪切应力（fluid shear stress）可使股动脉结扎兔的血流完全恢复正常。该效果可通过外科干预手段实现：于股动脉与其伴行静脉之间构建动静脉吻合术（arterio-venous anastomosis，即分流术）。本研究采用基因谱分析（gene profiling）方法，旨在对比分流模型中侧支生长阶段与预先存在的侧支（假手术组，sham），以筛选差异表达基因（differentially expressed genes）。